Primers Available
Organism:Halocynthia spinosa complete mitochondrial genome
Repeat:(T)11
Start:10110 End:10120
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCGAGATGTTGTTCGTGAGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCGGCCAACTACACCTTAAA | |||||||||||||
| Tm_F_1 | 59.992 | Size_F_1 | 20 | Tm_R_1 | 60.131 | Size_R_1 | 20 | PRODUCT Size | 179 bp | Primer Start | 10009 | Primer End | 10187 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTGCGTATTGGGATGGTTCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCGGCCAACTACACCTTAAA | |||||||||||||
| Tm_F_2 | 59.955 | Size_F_2 | 20 | Tm_R_2 | 60.131 | Size_R_2 | 20 | PRODUCT Size | 118 bp | Primer Start | 10070 | Primer End | 10187 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTGGTTCGTTGGTTTCATCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCGGCCAACTACACCTTAAA | |||||||||||||
| Tm_F_3 | 59.941 | Size_F_3 | 20 | Tm_R_3 | 60.131 | Size_R_3 | 20 | PRODUCT Size | 262 bp | Primer Start | 9926 | Primer End | 10187 |