Primers Available
Organism:Halocynthia spinosa complete mitochondrial genome
Repeat:(T)10atatcgtttatttgtgggtgttgtggtagttggtttgtttttgttaggtactgtgtttactttttctgaagaggttttg(T)11
Start:14598 End:14697
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTGGTGAGTGTTTGTGGACC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGCTACTTAACCCCACAGCA | |||||||||||||
| Tm_F_1 | 59.415 | Size_F_1 | 20 | Tm_R_1 | 59.342 | Size_R_1 | 20 | PRODUCT Size | 241 bp | Primer Start | 14527 | Primer End | 14767 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTCAGGAGGTTTGTTGGTGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGCTACTTAACCCCACAGCA | |||||||||||||
| Tm_F_2 | 59.109 | Size_F_2 | 20 | Tm_R_2 | 59.342 | Size_R_2 | 20 | PRODUCT Size | 254 bp | Primer Start | 14514 | Primer End | 14767 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGAAGATTATAGGCGGGTCTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACCCACAACGCACACTTATG | |||||||||||||
| Tm_F_3 | 60.068 | Size_F_3 | 21 | Tm_R_3 | 59.49 | Size_R_3 | 20 | PRODUCT Size | 274 bp | Primer Start | 14564 | Primer End | 14837 |