Primers Available
Organism:Halocynthia spinosa complete mitochondrial genome
Repeat:(T)10
Start:7769 End:7778
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTCTATTCTTCGGTGTGGGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAAAATCCGCTCTGGTTGTG | |||||||||||||
| Tm_F_1 | 60.074 | Size_F_1 | 20 | Tm_R_1 | 60.11 | Size_R_1 | 20 | PRODUCT Size | 113 bp | Primer Start | 7738 | Primer End | 7850 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTCTATTCTTCGGTGTGGGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CAAAAATCCGCTCTGGTTGT | |||||||||||||
| Tm_F_2 | 60.074 | Size_F_2 | 20 | Tm_R_2 | 60.11 | Size_R_2 | 20 | PRODUCT Size | 114 bp | Primer Start | 7738 | Primer End | 7851 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTCTATTCTTCGGTGTGGGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCCCACTAATTCAATCCCCA | |||||||||||||
| Tm_F_3 | 60.074 | Size_F_3 | 20 | Tm_R_3 | 60.126 | Size_R_3 | 20 | PRODUCT Size | 204 bp | Primer Start | 7738 | Primer End | 7941 |