Primers Available
Organism:Halocynthia spinosa complete mitochondrial genome
Repeat:(T)10
Start:5446 End:5455
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGTTAGTGACGGCACATGCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGCCAATGGTGGATAAACAG | |||||||||||||
| Tm_F_1 | 60.333 | Size_F_1 | 20 | Tm_R_1 | 60.192 | Size_R_1 | 20 | PRODUCT Size | 233 bp | Primer Start | 5415 | Primer End | 5647 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGTTAGTGACGGCACATGCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AATCCAAAGCAGGACCAGAA | |||||||||||||
| Tm_F_2 | 60.333 | Size_F_2 | 20 | Tm_R_2 | 59.67 | Size_R_2 | 20 | PRODUCT Size | 267 bp | Primer Start | 5415 | Primer End | 5681 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGTTAGTGACGGCACATGCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAATCCAAAGCAGGACCAGA | |||||||||||||
| Tm_F_3 | 60.333 | Size_F_3 | 20 | Tm_R_3 | 59.67 | Size_R_3 | 20 | PRODUCT Size | 268 bp | Primer Start | 5415 | Primer End | 5682 |