Primers Available
Organism:Halocynthia spinosa complete mitochondrial genome
Repeat:(T)10
Start:10346 End:10355
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGGGATTATCAGAGAGCGGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCAAAACATGAAACCCATGA | |||||||||||||
| Tm_F_1 | 60.431 | Size_F_1 | 20 | Tm_R_1 | 59.222 | Size_R_1 | 20 | PRODUCT Size | 169 bp | Primer Start | 10295 | Primer End | 10463 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGTGGGGGTGGAGGTATTAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCAAAACATGAAACCCATGA | |||||||||||||
| Tm_F_2 | 60.434 | Size_F_2 | 20 | Tm_R_2 | 59.222 | Size_R_2 | 20 | PRODUCT Size | 275 bp | Primer Start | 10189 | Primer End | 10463 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGGGTGGAGGTATTAGACCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCAAAACATGAAACCCATGA | |||||||||||||
| Tm_F_3 | 59.513 | Size_F_3 | 20 | Tm_R_3 | 59.222 | Size_R_3 | 20 | PRODUCT Size | 271 bp | Primer Start | 10193 | Primer End | 10463 |