Primers Available
Organism:Halocynthia spinosa complete mitochondrial genome
Repeat:(GTTT)3
Start:3207 End:3218
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCAGCTTGCTGCTGCTATTA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CACATTTCAACGCCACAAAC | |||||||||||||
| Tm_F_1 | 59.519 | Size_F_1 | 20 | Tm_R_1 | 60.011 | Size_R_1 | 20 | PRODUCT Size | 252 bp | Primer Start | 3069 | Primer End | 3320 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCAGCTTGCTGCTGCTATTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATAAGCCACATTTCAACGCC | |||||||||||||
| Tm_F_2 | 59.519 | Size_F_2 | 20 | Tm_R_2 | 59.967 | Size_R_2 | 20 | PRODUCT Size | 258 bp | Primer Start | 3069 | Primer End | 3326 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTGTTGGGGTATTGGTGAGAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CACATTTCAACGCCACAAAC | |||||||||||||
| Tm_F_3 | 59.838 | Size_F_3 | 21 | Tm_R_3 | 60.011 | Size_R_3 | 20 | PRODUCT Size | 185 bp | Primer Start | 3136 | Primer End | 3320 |