Primers Available
Organism:Halocynthia spinosa complete mitochondrial genome
Repeat:(G)12
Start:9614 End:9625
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TAGGGGCCTTGTTTGATTTG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTGCCCCCTAAAAACAACAG | |||||||||||||
| Tm_F_1 | 59.931 | Size_F_1 | 20 | Tm_R_1 | 59.968 | Size_R_1 | 20 | PRODUCT Size | 165 bp | Primer Start | 9504 | Primer End | 9668 |
| FORWARD_PRIMER_2 | |||||||||||||
| TAGGGGCCTTGTTTGATTTG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AACCAAACAGCATCTTTGCC | |||||||||||||
| Tm_F_2 | 59.931 | Size_F_2 | 20 | Tm_R_2 | 60.118 | Size_R_2 | 20 | PRODUCT Size | 180 bp | Primer Start | 9504 | Primer End | 9683 |
| FORWARD_PRIMER_3 | |||||||||||||
| TAGGGGCCTTGTTTGATTTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACGCTCTTTTCGAATTGCAT | |||||||||||||
| Tm_F_3 | 59.931 | Size_F_3 | 20 | Tm_R_3 | 59.851 | Size_R_3 | 20 | PRODUCT Size | 276 bp | Primer Start | 9504 | Primer End | 9779 |