Primers Available
Organism:Halocynthia spinosa complete mitochondrial genome
Repeat:(ATTT)3
Start:2803 End:2814
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GTTTTGGGTTTGAGCCGTAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AATACACCAGGTACAGCACCG | |||||||||||||
| Tm_F_1 | 59.975 | Size_F_1 | 20 | Tm_R_1 | 59.928 | Size_R_1 | 21 | PRODUCT Size | 173 bp | Primer Start | 2736 | Primer End | 2908 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTTTGTTTTGGGTTTGAGCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AATACACCAGGTACAGCACCG | |||||||||||||
| Tm_F_2 | 59.953 | Size_F_2 | 20 | Tm_R_2 | 59.928 | Size_R_2 | 21 | PRODUCT Size | 177 bp | Primer Start | 2732 | Primer End | 2908 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATAGGGGTTTTGGCTTTGGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AATACACCAGGTACAGCACCG | |||||||||||||
| Tm_F_3 | 59.704 | Size_F_3 | 20 | Tm_R_3 | 59.928 | Size_R_3 | 21 | PRODUCT Size | 277 bp | Primer Start | 2632 | Primer End | 2908 |