Primers Available
Organism:Pyura gangelion complete mitochondrial genome
Repeat:(T)18
Start:12063 End:12080
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACCTAAAGCACACGTGGAGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCCTCCTACAACGCTTAGCC | |||||||||||||
| Tm_F_1 | 60.171 | Size_F_1 | 20 | Tm_R_1 | 61.125 | Size_R_1 | 20 | PRODUCT Size | 160 bp | Primer Start | 11945 | Primer End | 12104 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACCTAAAGCACACGTGGAGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGACTTTACGTCGGACTGCC | |||||||||||||
| Tm_F_2 | 60.171 | Size_F_2 | 20 | Tm_R_2 | 61.236 | Size_R_2 | 20 | PRODUCT Size | 205 bp | Primer Start | 11945 | Primer End | 12149 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACCTAAAGCACACGTGGAGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACTGCCGCATGCAAACTAA | |||||||||||||
| Tm_F_3 | 60.171 | Size_F_3 | 20 | Tm_R_3 | 60.417 | Size_R_3 | 19 | PRODUCT Size | 191 bp | Primer Start | 11945 | Primer End | 12135 |