Primers Available
Organism:Pyura gangelion complete mitochondrial genome
Repeat:(T)12
Start:7584 End:7595
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATGCTAATTGTCGCAGGGTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGAAAAGGACTCAAACCCAC | |||||||||||||
| Tm_F_1 | 60.103 | Size_F_1 | 20 | Tm_R_1 | 59.569 | Size_R_1 | 20 | PRODUCT Size | 221 bp | Primer Start | 7521 | Primer End | 7741 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTCATGCTAATTGTCGCAGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGAAAAGGACTCAAACCCAC | |||||||||||||
| Tm_F_2 | 59.833 | Size_F_2 | 20 | Tm_R_2 | 59.569 | Size_R_2 | 20 | PRODUCT Size | 224 bp | Primer Start | 7518 | Primer End | 7741 |
| FORWARD_PRIMER_3 | |||||||||||||
| TAATTGTCGCAGGGTCTGTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGAAAAGGACTCAAACCCAC | |||||||||||||
| Tm_F_3 | 59.716 | Size_F_3 | 20 | Tm_R_3 | 59.569 | Size_R_3 | 20 | PRODUCT Size | 217 bp | Primer Start | 7525 | Primer End | 7741 |