Primers Available
Organism:Pyura gangelion complete mitochondrial genome
Repeat:(T)11
Start:12999 End:13009
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CAGGTTATTAGGGATGGGCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACCGAGGGAAAGCCATATCT | |||||||||||||
| Tm_F_1 | 59.778 | Size_F_1 | 20 | Tm_R_1 | 59.925 | Size_R_1 | 20 | PRODUCT Size | 154 bp | Primer Start | 12937 | Primer End | 13090 |
| FORWARD_PRIMER_2 | |||||||||||||
| CAGGTTATTAGGGATGGGCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AACCGAGGGAAAGCCATATC | |||||||||||||
| Tm_F_2 | 59.778 | Size_F_2 | 20 | Tm_R_2 | 60.286 | Size_R_2 | 20 | PRODUCT Size | 155 bp | Primer Start | 12937 | Primer End | 13091 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGGGCAGGTTATTAGGGATG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACCGAGGGAAAGCCATATCT | |||||||||||||
| Tm_F_3 | 60.522 | Size_F_3 | 20 | Tm_R_3 | 59.925 | Size_R_3 | 20 | PRODUCT Size | 158 bp | Primer Start | 12933 | Primer End | 13090 |