Primers Available
Organism:Botryllus schlosseri complete mitochondrial genome
Repeat:(T)10aagttttgttagatttaatttagattttttcttggaatttaatttatggttcaattatagatttttattgacttttgatgtttattctacta(T)14
Start:2601 End:2716
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGTGGAAATTAACAAGCTACAAAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TACCTACCCCGTTTTCATCG | |||||||||||||
| Tm_F_1 | 57.101 | Size_F_1 | 24 | Tm_R_1 | 59.817 | Size_R_1 | 20 | PRODUCT Size | 273 bp | Primer Start | 2513 | Primer End | 2785 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATGTGGAAATTAACAAGCTACAAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TACCTACCCCGTTTTCATCG | |||||||||||||
| Tm_F_2 | 57.101 | Size_F_2 | 24 | Tm_R_2 | 59.817 | Size_R_2 | 20 | PRODUCT Size | 274 bp | Primer Start | 2512 | Primer End | 2785 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTTTTATGTGGAAATTAACAAGCTACA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TACCTACCCCGTTTTCATCG | |||||||||||||
| Tm_F_3 | 59.482 | Size_F_3 | 27 | Tm_R_3 | 59.817 | Size_R_3 | 20 | PRODUCT Size | 279 bp | Primer Start | 2507 | Primer End | 2785 |