Primers Available
Organism:Paralithodes brevipes mitochondrial DNA
Repeat:(TAA)4
Start:474 End:485
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAGCGCAAATCCACCTCTAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTTTTGATTGGGGGTTTCAA | |||||||||||||
| Tm_F_1 | 59.34 | Size_F_1 | 20 | Tm_R_1 | 60.138 | Size_R_1 | 20 | PRODUCT Size | 204 bp | Primer Start | 338 | Primer End | 541 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCAAACATGAAGCCAACCAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCGGCTGATCTTCAAATTGT | |||||||||||||
| Tm_F_2 | 60.755 | Size_F_2 | 20 | Tm_R_2 | 60.074 | Size_R_2 | 20 | PRODUCT Size | 216 bp | Primer Start | 433 | Primer End | 648 |
| FORWARD_PRIMER_3 | |||||||||||||
| AATAAGCGCAAATCCACCTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTTTGATTGGGGGTTTCAA | |||||||||||||
| Tm_F_3 | 59.186 | Size_F_3 | 20 | Tm_R_3 | 60.138 | Size_R_3 | 20 | PRODUCT Size | 207 bp | Primer Start | 335 | Primer End | 541 |