Primers Available
Organism:Emberiza spodocephala mitochondrion
Repeat:(ACCA)3
Start:15378 End:15389
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACCAACCCTATCCCAAAACC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGTCTAACCCGTCCCCTTAT | |||||||||||||
| Tm_F_1 | 59.919 | Size_F_1 | 20 | Tm_R_1 | 60.2 | Size_R_1 | 20 | PRODUCT Size | 232 bp | Primer Start | 15218 | Primer End | 15449 |
| FORWARD_PRIMER_2 | |||||||||||||
| AACAAAAGGAACCCCCAAAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTATAACGGCGGCTTTTCAG | |||||||||||||
| Tm_F_2 | 60.068 | Size_F_2 | 20 | Tm_R_2 | 60.218 | Size_R_2 | 20 | PRODUCT Size | 208 bp | Primer Start | 15355 | Primer End | 15562 |
| FORWARD_PRIMER_3 | |||||||||||||
| AACAAAAGGAACCCCCAAAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGGGTTAGCTGTTGCGTCTA | |||||||||||||
| Tm_F_3 | 60.068 | Size_F_3 | 20 | Tm_R_3 | 60.27 | Size_R_3 | 20 | PRODUCT Size | 109 bp | Primer Start | 15355 | Primer End | 15463 |