Primers Available
Organism:Pinus massoniana chloroplast
Repeat:(T)18
Start:87321 End:87338
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCAAGTCCAGGATAGCCCAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATGTTTCGGTCCTATTCCCC | |||||||||||||
| Tm_F_1 | 60.21 | Size_F_1 | 20 | Tm_R_1 | 60.017 | Size_R_1 | 20 | PRODUCT Size | 269 bp | Primer Start | 87250 | Primer End | 87518 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCAAGTCCAGGATAGCCCAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTTTCGGTCCTATTCCCCAT | |||||||||||||
| Tm_F_2 | 60.21 | Size_F_2 | 20 | Tm_R_2 | 60.017 | Size_R_2 | 20 | PRODUCT Size | 267 bp | Primer Start | 87250 | Primer End | 87516 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGATGAATCGCTCCTGAAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATATCCCCCGTACTTGGACC | |||||||||||||
| Tm_F_3 | 60.155 | Size_F_3 | 20 | Tm_R_3 | 59.903 | Size_R_3 | 20 | PRODUCT Size | 226 bp | Primer Start | 87148 | Primer End | 87373 |