Primers Available
Organism:Pinus massoniana chloroplast
Repeat:(GAAA)3
Start:48827 End:48838
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATCTGGACCAACGTTTGCTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCCCACTCATCTCACAATGA | |||||||||||||
| Tm_F_1 | 59.598 | Size_F_1 | 20 | Tm_R_1 | 59.637 | Size_R_1 | 20 | PRODUCT Size | 254 bp | Primer Start | 48716 | Primer End | 48969 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATCTGGACCAACGTTTGCTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGAGCCCACTCATCTCACAA | |||||||||||||
| Tm_F_2 | 59.598 | Size_F_2 | 20 | Tm_R_2 | 60.402 | Size_R_2 | 20 | PRODUCT Size | 257 bp | Primer Start | 48716 | Primer End | 48972 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATGCAATCTACCGGCTATGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGGACAGATCTATTGCCGAG | |||||||||||||
| Tm_F_3 | 60.088 | Size_F_3 | 20 | Tm_R_3 | 60.755 | Size_R_3 | 20 | PRODUCT Size | 254 bp | Primer Start | 48683 | Primer End | 48936 |