Primers Available
Organism:Pinus massoniana chloroplast
Repeat:(AGGT)3
Start:90011 End:90022
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGCCATACTCCCAGGAAAAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGGAGACCTGTGTTTTTGGT | |||||||||||||
| Tm_F_1 | 60.066 | Size_F_1 | 20 | Tm_R_1 | 60.005 | Size_R_1 | 20 | PRODUCT Size | 218 bp | Primer Start | 89944 | Primer End | 90161 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGCCATACTCCCAGGAAAAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTTTTGGTAAACAGTCGCCC | |||||||||||||
| Tm_F_2 | 60.066 | Size_F_2 | 20 | Tm_R_2 | 59.975 | Size_R_2 | 20 | PRODUCT Size | 206 bp | Primer Start | 89944 | Primer End | 90149 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGCCATACTCCCAGGAAAAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCCCCATACATGGTCTTACG | |||||||||||||
| Tm_F_3 | 60.066 | Size_F_3 | 20 | Tm_R_3 | 60.066 | Size_R_3 | 20 | PRODUCT Size | 244 bp | Primer Start | 89944 | Primer End | 90187 |