Primers Available
Organism:Pinus massoniana chloroplast
Repeat:(A)10(G)10
Start:30275 End:30294
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCCAAGCTTATGGCATTGTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTGTATGCGGAATCAACTGG | |||||||||||||
| Tm_F_1 | 59.96 | Size_F_1 | 20 | Tm_R_1 | 59.542 | Size_R_1 | 20 | PRODUCT Size | 208 bp | Primer Start | 30159 | Primer End | 30366 |
| FORWARD_PRIMER_2 | |||||||||||||
| CAACCCGGAGAGGTAATTCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTGTATGCGGAATCAACTGG | |||||||||||||
| Tm_F_2 | 59.926 | Size_F_2 | 20 | Tm_R_2 | 59.542 | Size_R_2 | 20 | PRODUCT Size | 269 bp | Primer Start | 30098 | Primer End | 30366 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACGGGACCTGAACAGAGATG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTGTATGCGGAATCAACTGG | |||||||||||||
| Tm_F_3 | 60.112 | Size_F_3 | 20 | Tm_R_3 | 59.542 | Size_R_3 | 20 | PRODUCT Size | 249 bp | Primer Start | 30118 | Primer End | 30366 |