Primers Available
Organism:Pinus massoniana chloroplast
Repeat:(A)10
Start:9909 End:9918
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAGCTAACGATGCGGGTTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATCCATTAGACGATGGACGC | |||||||||||||
| Tm_F_1 | 60.228 | Size_F_1 | 19 | Tm_R_1 | 59.923 | Size_R_1 | 20 | PRODUCT Size | 280 bp | Primer Start | 9675 | Primer End | 9954 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCGCTACCCGCTAACAGAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATCCATTAGACGATGGACGC | |||||||||||||
| Tm_F_2 | 60.242 | Size_F_2 | 19 | Tm_R_2 | 59.923 | Size_R_2 | 20 | PRODUCT Size | 256 bp | Primer Start | 9699 | Primer End | 9954 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGATCGGGACAATGTATGCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCCAATCCAATACATTGCACA | |||||||||||||
| Tm_F_3 | 59.78 | Size_F_3 | 20 | Tm_R_3 | 59.801 | Size_R_3 | 21 | PRODUCT Size | 180 bp | Primer Start | 9873 | Primer End | 10052 |