Primers Available
Organism:Cymbidium mannii voucher KUN:YJB100602 chloroplast
Repeat:(T)12
Start:128343 End:128354
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCTTCTCCCAAATTCTCGGTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CACGTGCAATCATGGGTACT | |||||||||||||
| Tm_F_1 | 60.06 | Size_F_1 | 21 | Tm_R_1 | 59.445 | Size_R_1 | 20 | PRODUCT Size | 274 bp | Primer Start | 128251 | Primer End | 128524 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCTCTTCTCCCAAATTCTCG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CACGTGCAATCATGGGTACT | |||||||||||||
| Tm_F_2 | 58.859 | Size_F_2 | 20 | Tm_R_2 | 59.445 | Size_R_2 | 20 | PRODUCT Size | 276 bp | Primer Start | 128249 | Primer End | 128524 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCTTCTCCCAAATTCTCGGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CACGTGCAATCATGGGTACT | |||||||||||||
| Tm_F_3 | 58.715 | Size_F_3 | 20 | Tm_R_3 | 59.445 | Size_R_3 | 20 | PRODUCT Size | 274 bp | Primer Start | 128251 | Primer End | 128524 |