Primers Available
Organism:Cymbidium tortisepalum voucher KUN:HJL091027 chloroplast
Repeat:(ATA)4
Start:3492 End:3503
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TATGGTCAGAACGGCGTATG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCTCTCTGTCTCCGGGGTAT | |||||||||||||
| Tm_F_1 | 59.566 | Size_F_1 | 20 | Tm_R_1 | 60.617 | Size_R_1 | 20 | PRODUCT Size | 196 bp | Primer Start | 3399 | Primer End | 3594 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAAGGGAGGCACTTCTTGTG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCTCTCTGTCTCCGGGGTAT | |||||||||||||
| Tm_F_2 | 59.328 | Size_F_2 | 20 | Tm_R_2 | 60.617 | Size_R_2 | 20 | PRODUCT Size | 239 bp | Primer Start | 3356 | Primer End | 3594 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGGCGTATGCATATGTTGTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCTCTCTGTCTCCGGGGTAT | |||||||||||||
| Tm_F_3 | 60.947 | Size_F_3 | 20 | Tm_R_3 | 60.617 | Size_R_3 | 20 | PRODUCT Size | 185 bp | Primer Start | 3410 | Primer End | 3594 |