Primers Available
Organism:Cymbidium tortisepalum voucher KUN:HJL091027 chloroplast
Repeat:(AAATAT)3aaaaatataaataaaaatataaatatataaa(AT)8
Start:4703 End:4767
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGGGTCCACAAAAATAAGCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAAAAGAAAGGAAAAACAAGAATCA | |||||||||||||
| Tm_F_1 | 59.801 | Size_F_1 | 20 | Tm_R_1 | 58.858 | Size_R_1 | 25 | PRODUCT Size | 262 bp | Primer Start | 4676 | Primer End | 4937 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGGGTCCACAAAAATAAGCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAAAGAAAGGAAAAACAAGAATCA | |||||||||||||
| Tm_F_2 | 59.801 | Size_F_2 | 20 | Tm_R_2 | 57.68 | Size_R_2 | 24 | PRODUCT Size | 261 bp | Primer Start | 4676 | Primer End | 4936 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGGGTCCACAAAAATAAGCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTCAAAGTTTCCTCTTTTCTTTTTC | |||||||||||||
| Tm_F_3 | 59.801 | Size_F_3 | 20 | Tm_R_3 | 58.644 | Size_R_3 | 25 | PRODUCT Size | 179 bp | Primer Start | 4676 | Primer End | 4854 |