Primers Available
Organism:Cymbidium sinense voucher KUN:YJB100605 chloroplast
Repeat:(AT)6
Start:99 End:110
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTGGGGGATTGCTATCTTCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGCACTATGGCGTTCAACCT | |||||||||||||
| Tm_F_1 | 60.405 | Size_F_1 | 20 | Tm_R_1 | 59.763 | Size_R_1 | 20 | PRODUCT Size | 267 bp | Primer Start | 57 | Primer End | 323 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTGGGGGATTGCTATCTTCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CACTATGGCGTTCAACCTGA | |||||||||||||
| Tm_F_2 | 60.405 | Size_F_2 | 20 | Tm_R_2 | 59.716 | Size_R_2 | 20 | PRODUCT Size | 265 bp | Primer Start | 57 | Primer End | 321 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTGGGGGATTGCTATCTTCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCATGAACGTAATGCACACA | |||||||||||||
| Tm_F_3 | 60.405 | Size_F_3 | 20 | Tm_R_3 | 59.155 | Size_R_3 | 20 | PRODUCT Size | 145 bp | Primer Start | 57 | Primer End | 201 |