Primers Available
Organism:Cymbidium aloifolium voucher KUN:YJB100604 chloroplast
Repeat:(TCATA)3taattc(AT)6
Start:100 End:132
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTGGGGGATTGCTATCTTCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AATGCACGAACGTAATGCAC | |||||||||||||
| Tm_F_1 | 60.405 | Size_F_1 | 20 | Tm_R_1 | 59.62 | Size_R_1 | 20 | PRODUCT Size | 170 bp | Primer Start | 57 | Primer End | 226 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACAAGATAGCAATCCCCCAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AATGCACGAACGTAATGCAC | |||||||||||||
| Tm_F_2 | 59.387 | Size_F_2 | 20 | Tm_R_2 | 59.62 | Size_R_2 | 20 | PRODUCT Size | 215 bp | Primer Start | 12 | Primer End | 226 |
| FORWARD_PRIMER_3 | |||||||||||||
| AACAAGATAGCAATCCCCCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AATGCACGAACGTAATGCAC | |||||||||||||
| Tm_F_3 | 59.387 | Size_F_3 | 20 | Tm_R_3 | 59.62 | Size_R_3 | 20 | PRODUCT Size | 216 bp | Primer Start | 11 | Primer End | 226 |