Primers Available
Organism:Tetracentron sinense chloroplast
Repeat:(A)22tcagagaagaagaaatcc(T)14
Start:72886 End:72939
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCGAAAAATCCGAGAATCCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGGAATGATTGAAACGGAAT | |||||||||||||
| Tm_F_1 | 60.523 | Size_F_1 | 20 | Tm_R_1 | 59.762 | Size_R_1 | 20 | PRODUCT Size | 206 bp | Primer Start | 72838 | Primer End | 73043 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCAACCCGAACTCAAACTCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGGAATGATTGAAACGGAAT | |||||||||||||
| Tm_F_2 | 59.262 | Size_F_2 | 20 | Tm_R_2 | 59.762 | Size_R_2 | 20 | PRODUCT Size | 240 bp | Primer Start | 72804 | Primer End | 73043 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTCGAAAAATCCGAGAATCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGGAATGATTGAAACGGAAT | |||||||||||||
| Tm_F_3 | 59.091 | Size_F_3 | 20 | Tm_R_3 | 59.762 | Size_R_3 | 20 | PRODUCT Size | 207 bp | Primer Start | 72837 | Primer End | 73043 |