Primers Available
Organism:Gymnocypris dobula voucher ECSFRI-GYDO01 mitochondrion
Repeat:(TA)12
Start:16595 End:16618
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGGAGGACCCGAGAACGTAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GTGTCGAGCCCCTTTTTACA | |||||||||||||
| Tm_F_1 | 59.957 | Size_F_1 | 20 | Tm_R_1 | 60.11 | Size_R_1 | 20 | PRODUCT Size | 160 bp | Primer Start | 16537 | Primer End | 16696 |
| FORWARD_PRIMER_2 | |||||||||||||
| AACCCCTAAAGCAAGGAGGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTGTCGAGCCCCTTTTTACA | |||||||||||||
| Tm_F_2 | 60.068 | Size_F_2 | 20 | Tm_R_2 | 60.11 | Size_R_2 | 20 | PRODUCT Size | 173 bp | Primer Start | 16524 | Primer End | 16696 |
| FORWARD_PRIMER_3 | |||||||||||||
| GAGGACCCGAGAACGTATCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTGTCGAGCCCCTTTTTACA | |||||||||||||
| Tm_F_3 | 60.073 | Size_F_3 | 20 | Tm_R_3 | 60.11 | Size_R_3 | 20 | PRODUCT Size | 158 bp | Primer Start | 16539 | Primer End | 16696 |