Primers Available
Organism:Gymnocypris dobula voucher ECSFRI-GYDO01 mitochondrion
Repeat:(AACA)3
Start:13488 End:13499
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CAAAACTTCCTCCCCATGAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGTCCAAGGGTAAGTTTGGC | |||||||||||||
| Tm_F_1 | 59.903 | Size_F_1 | 20 | Tm_R_1 | 59.971 | Size_R_1 | 20 | PRODUCT Size | 233 bp | Primer Start | 13374 | Primer End | 13606 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAACTTCCTCCCCATGAAGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGTCCAAGGGTAAGTTTGGC | |||||||||||||
| Tm_F_2 | 60.298 | Size_F_2 | 20 | Tm_R_2 | 59.971 | Size_R_2 | 20 | PRODUCT Size | 231 bp | Primer Start | 13376 | Primer End | 13606 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAAGATAGCAGCCCTTGCAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGTCCAAGGGTAAGTTTGGC | |||||||||||||
| Tm_F_3 | 59.615 | Size_F_3 | 20 | Tm_R_3 | 59.971 | Size_R_3 | 20 | PRODUCT Size | 183 bp | Primer Start | 13424 | Primer End | 13606 |