Primers Available
Organism:Protobothrops mucrosquamatus mitochondrion
Repeat:(ACA)4
Start:3447 End:3458
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTCCTATGAATCCGTGCCTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GACCCTCTTTGATGTGGGAA | |||||||||||||
| Tm_F_1 | 60.036 | Size_F_1 | 20 | Tm_R_1 | 59.903 | Size_R_1 | 20 | PRODUCT Size | 229 bp | Primer Start | 3335 | Primer End | 3563 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCCACGATTTCGATATGACC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GACCCTCTTTGATGTGGGAA | |||||||||||||
| Tm_F_2 | 60.155 | Size_F_2 | 20 | Tm_R_2 | 59.903 | Size_R_2 | 20 | PRODUCT Size | 206 bp | Primer Start | 3358 | Primer End | 3563 |
| FORWARD_PRIMER_3 | |||||||||||||
| GTGCCTCATACCCACGATTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GACCCTCTTTGATGTGGGAA | |||||||||||||
| Tm_F_3 | 59.82 | Size_F_3 | 20 | Tm_R_3 | 59.903 | Size_R_3 | 20 | PRODUCT Size | 216 bp | Primer Start | 3348 | Primer End | 3563 |