Primers Available
Organism:Emberiza pusilla mitochondrion
Repeat:(C)13
Start:954 End:966
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAGTGGGATAATCGAGCCCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GTGCACCTTCCGGTACACTT | |||||||||||||
| Tm_F_1 | 59.925 | Size_F_1 | 20 | Tm_R_1 | 60.035 | Size_R_1 | 20 | PRODUCT Size | 156 bp | Primer Start | 879 | Primer End | 1034 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAGTGGGATAATCGAGCCCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACCTCATCTTTGGCTTGGTG | |||||||||||||
| Tm_F_2 | 59.925 | Size_F_2 | 20 | Tm_R_2 | 60.111 | Size_R_2 | 20 | PRODUCT Size | 121 bp | Primer Start | 879 | Primer End | 999 |
| FORWARD_PRIMER_3 | |||||||||||||
| AATCGAGCCCTCTTTAAGCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTGCACCTTCCGGTACACTT | |||||||||||||
| Tm_F_3 | 59.82 | Size_F_3 | 20 | Tm_R_3 | 60.035 | Size_R_3 | 20 | PRODUCT Size | 147 bp | Primer Start | 888 | Primer End | 1034 |