Primers Available
Organism:Scomberomorus semifasciatus strain GREY-SsPD211135 mitochondrion
Repeat:(TCCT)3
Start:7938 End:7949
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCATTTCCCCGAATAAACAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GAGATGGCAGGGGGTTTTAT | |||||||||||||
| Tm_F_1 | 59.773 | Size_F_1 | 20 | Tm_R_1 | 60.152 | Size_R_1 | 20 | PRODUCT Size | 254 bp | Primer Start | 7896 | Primer End | 8149 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCATTTCCCCGAATAAACAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGAACAGGGGTGTCTGGTAT | |||||||||||||
| Tm_F_2 | 59.773 | Size_F_2 | 20 | Tm_R_2 | 59.844 | Size_R_2 | 20 | PRODUCT Size | 277 bp | Primer Start | 7896 | Primer End | 8172 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCATTTCCCCGAATAAACAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATGGTTAGGTCAACGGATGC | |||||||||||||
| Tm_F_3 | 59.773 | Size_F_3 | 20 | Tm_R_3 | 59.82 | Size_R_3 | 20 | PRODUCT Size | 164 bp | Primer Start | 7896 | Primer End | 8059 |