Primers Available
Organism:Scomberomorus munroi strain SPOTTY-09SPM0056 mitochondrion
Repeat:(ACA)4
Start:9406 End:9417
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATTGGGTCGCTAGTTTCCCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GTGGCTAATGTGGTTGGCTT | |||||||||||||
| Tm_F_1 | 59.962 | Size_F_1 | 20 | Tm_R_1 | 60 | Size_R_1 | 20 | PRODUCT Size | 250 bp | Primer Start | 9227 | Primer End | 9476 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCATTGGGTCGCTAGTTTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTGGCTAATGTGGTTGGCTT | |||||||||||||
| Tm_F_2 | 60.074 | Size_F_2 | 20 | Tm_R_2 | 60 | Size_R_2 | 20 | PRODUCT Size | 253 bp | Primer Start | 9224 | Primer End | 9476 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTACCACACCTTCGAGGAGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTGGCTAATGTGGTTGGCTT | |||||||||||||
| Tm_F_3 | 59.867 | Size_F_3 | 20 | Tm_R_3 | 60 | Size_R_3 | 20 | PRODUCT Size | 113 bp | Primer Start | 9364 | Primer End | 9476 |