Primers Available
Organism:Flammulina velutipes strain 4019-20 mitochondrion
Repeat:(TTTTAA)3
Start:3643 End:3660
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TACTCGATGCGGGAAACTCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGCCTTACGTTAAGTCTCTGATG | |||||||||||||
| Tm_F_1 | 59.836 | Size_F_1 | 20 | Tm_R_1 | 58.599 | Size_R_1 | 23 | PRODUCT Size | 182 bp | Primer Start | 3517 | Primer End | 3698 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTACTCGATGCGGGAAACTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGCCTTACGTTAAGTCTCTGATG | |||||||||||||
| Tm_F_2 | 60.214 | Size_F_2 | 20 | Tm_R_2 | 58.599 | Size_R_2 | 23 | PRODUCT Size | 183 bp | Primer Start | 3516 | Primer End | 3698 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGATGCGGGAAACTCTTCTA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGCCTTACGTTAAGTCTCTGATG | |||||||||||||
| Tm_F_3 | 60.344 | Size_F_3 | 20 | Tm_R_3 | 58.599 | Size_R_3 | 23 | PRODUCT Size | 178 bp | Primer Start | 3521 | Primer End | 3698 |