Primers Available
Organism:Flammulina velutipes strain 4019-20 mitochondrion
Repeat:(TTATTT)3
Start:60285 End:60302
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGTCAATCTCTGCCTGCCAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GTGTGTCGGGGGTTGATAAT | |||||||||||||
| Tm_F_1 | 59.834 | Size_F_1 | 20 | Tm_R_1 | 59.532 | Size_R_1 | 20 | PRODUCT Size | 139 bp | Primer Start | 60198 | Primer End | 60336 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATCTCTGCCTGCCATACGAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTGTGTCGGGGGTTGATAAT | |||||||||||||
| Tm_F_2 | 60.249 | Size_F_2 | 20 | Tm_R_2 | 59.532 | Size_R_2 | 20 | PRODUCT Size | 134 bp | Primer Start | 60203 | Primer End | 60336 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGTCAATCTCTGCCTGCCAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTGTCGGGGGTTGATAATTC | |||||||||||||
| Tm_F_3 | 59.834 | Size_F_3 | 20 | Tm_R_3 | 59.105 | Size_R_3 | 20 | PRODUCT Size | 137 bp | Primer Start | 60198 | Primer End | 60334 |