Primers Available
Organism:Flammulina velutipes strain 4019-20 mitochondrion
Repeat:(T)10
Start:65663 End:65672
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAAACATTCTGATGGTGCAGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GAATTGTTGAAATAGTTCCAAGACC | |||||||||||||
| Tm_F_1 | 57.186 | Size_F_1 | 21 | Tm_R_1 | 59.356 | Size_R_1 | 25 | PRODUCT Size | 260 bp | Primer Start | 65515 | Primer End | 65774 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAAACATTCTGATGGTGCAGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTGTTGAAATAGTTCCAAGACCT | |||||||||||||
| Tm_F_2 | 57.186 | Size_F_2 | 21 | Tm_R_2 | 57.008 | Size_R_2 | 23 | PRODUCT Size | 257 bp | Primer Start | 65515 | Primer End | 65771 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAAACATTCTGATGGTGCAGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGAATTGTTGAAATAGTTCCAAGACC | |||||||||||||
| Tm_F_3 | 57.186 | Size_F_3 | 21 | Tm_R_3 | 60.161 | Size_R_3 | 26 | PRODUCT Size | 261 bp | Primer Start | 65515 | Primer End | 65775 |