Primers Available
Organism:Flammulina velutipes strain 4019-20 mitochondrion
Repeat:(AATT)3
Start:22029 End:22040
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCCTCTAGATACTACAAACGAATCTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGCGTTTACATTTGCAGCCT | |||||||||||||
| Tm_F_1 | 57.746 | Size_F_1 | 26 | Tm_R_1 | 59.911 | Size_R_1 | 20 | PRODUCT Size | 258 bp | Primer Start | 21918 | Primer End | 22175 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCCTCTAGATACTACAAACGAATCTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAGCGTTTACATTTGCAGCC | |||||||||||||
| Tm_F_2 | 57.746 | Size_F_2 | 26 | Tm_R_2 | 60.272 | Size_R_2 | 20 | PRODUCT Size | 259 bp | Primer Start | 21918 | Primer End | 22176 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACCCTCTAGATACTACAAACGAATCTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGCGTTTACATTTGCAGCCT | |||||||||||||
| Tm_F_3 | 58.461 | Size_F_3 | 27 | Tm_R_3 | 59.911 | Size_R_3 | 20 | PRODUCT Size | 259 bp | Primer Start | 21917 | Primer End | 22175 |