Primers Available
Organism:Flammulina velutipes strain 4019-20 mitochondrion
Repeat:(AATT)3
Start:1700 End:1711
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AATATTGGGCAATCAGCAGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTTCCAAAAGGGCTGGACTA | |||||||||||||
| Tm_F_1 | 59.923 | Size_F_1 | 20 | Tm_R_1 | 59.679 | Size_R_1 | 20 | PRODUCT Size | 259 bp | Primer Start | 1506 | Primer End | 1764 |
| FORWARD_PRIMER_2 | |||||||||||||
| AATATTGGGCAATCAGCAGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTTTCCAAAAGGGCTGGACT | |||||||||||||
| Tm_F_2 | 59.923 | Size_F_2 | 20 | Tm_R_2 | 60.476 | Size_R_2 | 20 | PRODUCT Size | 260 bp | Primer Start | 1506 | Primer End | 1765 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATATTGGGCAATCAGCAGGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTCCAAAAGGGCTGGACTA | |||||||||||||
| Tm_F_3 | 60.435 | Size_F_3 | 20 | Tm_R_3 | 59.679 | Size_R_3 | 20 | PRODUCT Size | 258 bp | Primer Start | 1507 | Primer End | 1764 |