Primers Available
Organism:Flammulina velutipes strain 4019-20 mitochondrion
Repeat:(AAT)4
Start:74870 End:74881
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CAGAATCACAAAATTCAAATGAAGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAAGAAGGGTTTAAGATTAAAGAGGG | |||||||||||||
| Tm_F_1 | 59.549 | Size_F_1 | 25 | Tm_R_1 | 59.837 | Size_R_1 | 26 | PRODUCT Size | 241 bp | Primer Start | 74801 | Primer End | 75041 |
| FORWARD_PRIMER_2 | |||||||||||||
| CAGAATCACAAAATTCAAATGAAGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAGAAGGGTTTAAGATTAAAGAGGG | |||||||||||||
| Tm_F_2 | 59.549 | Size_F_2 | 25 | Tm_R_2 | 58.793 | Size_R_2 | 25 | PRODUCT Size | 240 bp | Primer Start | 74801 | Primer End | 75040 |
| FORWARD_PRIMER_3 | |||||||||||||
| CAGAATCACAAAATTCAAATGAAGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GAAGGGTTTAAGATTAAAGAGGGTTT | |||||||||||||
| Tm_F_3 | 59.549 | Size_F_3 | 25 | Tm_R_3 | 59.737 | Size_R_3 | 26 | PRODUCT Size | 238 bp | Primer Start | 74801 | Primer End | 75038 |