Primers Available
Organism:Apalone spinifera mitochondrion
Repeat:(CTA)4
Start:8603 End:8614
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCATCCCAGCCCTCATTATC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATTAATGCAACGGCCAATTC | |||||||||||||
| Tm_F_1 | 59.854 | Size_F_1 | 20 | Tm_R_1 | 59.801 | Size_R_1 | 20 | PRODUCT Size | 217 bp | Primer Start | 8426 | Primer End | 8642 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCCAGCCCTCATTATCATC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATTAATGCAACGGCCAATTC | |||||||||||||
| Tm_F_2 | 59.854 | Size_F_2 | 20 | Tm_R_2 | 59.801 | Size_R_2 | 20 | PRODUCT Size | 214 bp | Primer Start | 8429 | Primer End | 8642 |
| FORWARD_PRIMER_3 | |||||||||||||
| AACAGCTAACCTGACAGCCG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCAGTTAGTGGTCATGGGCT | |||||||||||||
| Tm_F_3 | 60.453 | Size_F_3 | 20 | Tm_R_3 | 59.989 | Size_R_3 | 20 | PRODUCT Size | 260 bp | Primer Start | 8496 | Primer End | 8755 |