Primers Available
Organism:Apalone spinifera mitochondrion
Repeat:(ATTA)3
Start:16343 End:16354
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCTCAGGCCCACATACATGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GATTTTGGGGTTTGACGAGA | |||||||||||||
| Tm_F_1 | 60.072 | Size_F_1 | 20 | Tm_R_1 | 59.91 | Size_R_1 | 20 | PRODUCT Size | 237 bp | Primer Start | 16256 | Primer End | 16492 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGTTCAAATTGCTTGGATCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GATTTTGGGGTTTGACGAGA | |||||||||||||
| Tm_F_2 | 59.664 | Size_F_2 | 20 | Tm_R_2 | 59.91 | Size_R_2 | 20 | PRODUCT Size | 188 bp | Primer Start | 16305 | Primer End | 16492 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCTCAGGCCCACATACATGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATTCTCGGATTTTGGGGTTT | |||||||||||||
| Tm_F_3 | 60.072 | Size_F_3 | 20 | Tm_R_3 | 59.644 | Size_R_3 | 20 | PRODUCT Size | 244 bp | Primer Start | 16256 | Primer End | 16499 |