Primers Available
Organism:Apalone spinifera mitochondrion
Repeat:(AAC)4
Start:348 End:359
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CACCCCCAAGGGTACTACAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCGTGGGTTCAAGTTGTCTG | |||||||||||||
| Tm_F_1 | 59.705 | Size_F_1 | 20 | Tm_R_1 | 59.72 | Size_R_1 | 20 | PRODUCT Size | 271 bp | Primer Start | 219 | Primer End | 489 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCCCCAAGGGTACTACAACA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCGTGGGTTCAAGTTGTCTG | |||||||||||||
| Tm_F_2 | 59.705 | Size_F_2 | 20 | Tm_R_2 | 59.72 | Size_R_2 | 20 | PRODUCT Size | 269 bp | Primer Start | 221 | Primer End | 489 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCCCCAAGGGTACTACAACA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTCTTAGCGATCGTGGGTTC | |||||||||||||
| Tm_F_3 | 59.705 | Size_F_3 | 20 | Tm_R_3 | 59.7 | Size_R_3 | 20 | PRODUCT Size | 279 bp | Primer Start | 221 | Primer End | 499 |