Primers Available
Organism:Phrynocephalus mystaceus mitochondrion
Repeat:(ACA)4
Start:5404 End:5415
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GTCAACCAAACACAACTGCG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GTATGAAGCCTGTGAAGGGG | |||||||||||||
| Tm_F_1 | 60.199 | Size_F_1 | 20 | Tm_R_1 | 59.55 | Size_R_1 | 20 | PRODUCT Size | 277 bp | Primer Start | 5199 | Primer End | 5475 |
| FORWARD_PRIMER_2 | |||||||||||||
| GTCAACCAAACACAACTGCG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGTATGAAGCCTGTGAAGGG | |||||||||||||
| Tm_F_2 | 60.199 | Size_F_2 | 20 | Tm_R_2 | 59.55 | Size_R_2 | 20 | PRODUCT Size | 278 bp | Primer Start | 5199 | Primer End | 5476 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCAAACACAACTGCGAAAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTATGAAGCCTGTGAAGGGG | |||||||||||||
| Tm_F_3 | 59.746 | Size_F_3 | 20 | Tm_R_3 | 59.55 | Size_R_3 | 20 | PRODUCT Size | 272 bp | Primer Start | 5204 | Primer End | 5475 |