Primers Available
Organism:Bursaphelenchus mucronatus mitochondrion
Repeat:(T)13
Start:4281 End:4293
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCTTTAACTAATCGTTTAGGTGATTTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCAACTTCTAAAAGGAAACTGAGC | |||||||||||||
| Tm_F_1 | 57.403 | Size_F_1 | 27 | Tm_R_1 | 59.839 | Size_R_1 | 24 | PRODUCT Size | 165 bp | Primer Start | 4247 | Primer End | 4411 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCTTTAACTAATCGTTTAGGTGATTTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCTCTTACAGGAGTAGGAGCACTT | |||||||||||||
| Tm_F_2 | 57.403 | Size_F_2 | 27 | Tm_R_2 | 59.649 | Size_R_2 | 24 | PRODUCT Size | 203 bp | Primer Start | 4247 | Primer End | 4449 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTTTAACTAATCGTTTAGGTGATTTTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCAACTTCTAAAAGGAAACTGAGC | |||||||||||||
| Tm_F_3 | 57.07 | Size_F_3 | 27 | Tm_R_3 | 59.839 | Size_R_3 | 24 | PRODUCT Size | 164 bp | Primer Start | 4248 | Primer End | 4411 |