Primers Available
Organism:Bursaphelenchus mucronatus mitochondrion
Repeat:(T)10atatttttagaatgaagaactttaccttttaaatttaattttattaattttcaattttcttttttatttcttttatgcttagtaggagtaacagtttatt(TTA)4
Start:7405 End:7526
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGGGGTTCTTCAAGCTGGTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCTAGAGCGAATAGCACCTAAAAA | |||||||||||||
| Tm_F_1 | 60.11 | Size_F_1 | 20 | Tm_R_1 | 58.839 | Size_R_1 | 24 | PRODUCT Size | 280 bp | Primer Start | 7299 | Primer End | 7578 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGGGGTTCTTCAAGCTGGTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCTAGAGCGAATAGCACCTAAAA | |||||||||||||
| Tm_F_2 | 60.11 | Size_F_2 | 20 | Tm_R_2 | 57.612 | Size_R_2 | 23 | PRODUCT Size | 280 bp | Primer Start | 7299 | Primer End | 7578 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGGGTTCTTCAAGCTGGTTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTCTAGAGCGAATAGCACCTAAAAA | |||||||||||||
| Tm_F_3 | 60.476 | Size_F_3 | 20 | Tm_R_3 | 59.96 | Size_R_3 | 25 | PRODUCT Size | 280 bp | Primer Start | 7300 | Primer End | 7579 |