Primers Available
Organism:Pleodorina starrii plastid
Repeat:(G)12
Start:82403 End:82414
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GACGAAAGGCGAAACCATAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGGGGTGCTACTATTTGCCA | |||||||||||||
| Tm_F_1 | 60.074 | Size_F_1 | 20 | Tm_R_1 | 59.592 | Size_R_1 | 20 | PRODUCT Size | 227 bp | Primer Start | 82248 | Primer End | 82474 |
| FORWARD_PRIMER_2 | |||||||||||||
| GACGAAAGGCGAAACCATAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCTGATGACATGTCGCTCTC | |||||||||||||
| Tm_F_2 | 60.074 | Size_F_2 | 20 | Tm_R_2 | 59.537 | Size_R_2 | 20 | PRODUCT Size | 280 bp | Primer Start | 82248 | Primer End | 82527 |
| FORWARD_PRIMER_3 | |||||||||||||
| GAGCCATTGGCAAAACAAAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCCAAACTAGAGCACACACG | |||||||||||||
| Tm_F_3 | 59.945 | Size_F_3 | 20 | Tm_R_3 | 59.515 | Size_R_3 | 20 | PRODUCT Size | 272 bp | Primer Start | 82187 | Primer End | 82458 |