Primers Available
Organism:Salamandrella tridactyla isolate SS77 mitochondrion
Repeat:(TAAAG)3
Start:2207 End:2221
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GTGCAGAAGCGGGAATAAAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGATTGCGCTGTTATCCCTA | |||||||||||||
| Tm_F_1 | 60.209 | Size_F_1 | 20 | Tm_R_1 | 60.06 | Size_R_1 | 20 | PRODUCT Size | 276 bp | Primer Start | 2116 | Primer End | 2391 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGCAGAAGCGGGAATAAAAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGATTGCGCTGTTATCCCTA | |||||||||||||
| Tm_F_2 | 59.682 | Size_F_2 | 20 | Tm_R_2 | 60.06 | Size_R_2 | 20 | PRODUCT Size | 275 bp | Primer Start | 2117 | Primer End | 2391 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGACCGGTATGAATGGCAAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTCGCCCCAACCTAAAATTC | |||||||||||||
| Tm_F_3 | 59.955 | Size_F_3 | 20 | Tm_R_3 | 60.673 | Size_R_3 | 20 | PRODUCT Size | 205 bp | Primer Start | 2047 | Primer End | 2251 |