Primers Available
Organism:Salamandrella tridactyla isolate SS77 mitochondrion
Repeat:(AAT)4
Start:4656 End:4667
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCATTGCACACATAGGGTGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GAGGCAGTCCGCCTAAAGAT | |||||||||||||
| Tm_F_1 | 60.39 | Size_F_1 | 20 | Tm_R_1 | 60.733 | Size_R_1 | 20 | PRODUCT Size | 219 bp | Primer Start | 4477 | Primer End | 4695 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGGGCTAAACCAAACACAAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAGGCAGTCCGCCTAAAGAT | |||||||||||||
| Tm_F_2 | 60.399 | Size_F_2 | 20 | Tm_R_2 | 60.733 | Size_R_2 | 20 | PRODUCT Size | 263 bp | Primer Start | 4433 | Primer End | 4695 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCATTGCACACATAGGGTGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TAAATCCCGATATGGGAGGC | |||||||||||||
| Tm_F_3 | 60.39 | Size_F_3 | 20 | Tm_R_3 | 60.975 | Size_R_3 | 20 | PRODUCT Size | 234 bp | Primer Start | 4477 | Primer End | 4710 |