Primers Available
Organism:Naegleria fowleri mitochondrion
Repeat:(TTTTA)3
Start:23774 End:23788
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AATTTTCCTATTCCGGCGAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCGAAGAATAAAACGATAATCTTCCT | |||||||||||||
| Tm_F_1 | 59.772 | Size_F_1 | 20 | Tm_R_1 | 59.912 | Size_R_1 | 26 | PRODUCT Size | 196 bp | Primer Start | 23643 | Primer End | 23838 |
| FORWARD_PRIMER_2 | |||||||||||||
| AATTTTCCTATTCCGGCGAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CATTACAAAATCGAAGAATAAAACGA | |||||||||||||
| Tm_F_2 | 59.772 | Size_F_2 | 20 | Tm_R_2 | 59.83 | Size_R_2 | 26 | PRODUCT Size | 206 bp | Primer Start | 23643 | Primer End | 23848 |
| FORWARD_PRIMER_3 | |||||||||||||
| TAATTTTCCTATTCCGGCGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCGAAGAATAAAACGATAATCTTCCT | |||||||||||||
| Tm_F_3 | 59.521 | Size_F_3 | 20 | Tm_R_3 | 59.912 | Size_R_3 | 26 | PRODUCT Size | 197 bp | Primer Start | 23642 | Primer End | 23838 |