Primers Available
Organism:Naegleria fowleri mitochondrion
Repeat:(TTTA)3
Start:39512 End:39523
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GAAGCATGGAGAGAAGGACG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGAGTAGCTTGACGTTTTTCTTTTT | |||||||||||||
| Tm_F_1 | 59.95 | Size_F_1 | 20 | Tm_R_1 | 59.89 | Size_R_1 | 25 | PRODUCT Size | 278 bp | Primer Start | 39400 | Primer End | 39677 |
| FORWARD_PRIMER_2 | |||||||||||||
| GAAGCATGGAGAGAAGGACG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATGAGTAGCTTGACGTTTTTCTTTTT | |||||||||||||
| Tm_F_2 | 59.95 | Size_F_2 | 20 | Tm_R_2 | 60.112 | Size_R_2 | 26 | PRODUCT Size | 279 bp | Primer Start | 39400 | Primer End | 39678 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGGAGAGAAGGACGTACAGTTTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCCATCTATGAGTAGCTTGACG | |||||||||||||
| Tm_F_3 | 59.803 | Size_F_3 | 23 | Tm_R_3 | 58.882 | Size_R_3 | 22 | PRODUCT Size | 280 bp | Primer Start | 39406 | Primer End | 39685 |