Primers Available
Organism:Naegleria fowleri mitochondrion
Repeat:(TTTA)3
Start:18583 End:18594
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCAGGTACTGATTTTTGGGGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCCATACCAACGGTACTTGTCT | |||||||||||||
| Tm_F_1 | 60.235 | Size_F_1 | 21 | Tm_R_1 | 60.165 | Size_R_1 | 22 | PRODUCT Size | 157 bp | Primer Start | 18482 | Primer End | 18638 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGTGCAGGTACTGATTTTTGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCCATACCAACGGTACTTGTCT | |||||||||||||
| Tm_F_2 | 59.485 | Size_F_2 | 21 | Tm_R_2 | 60.165 | Size_R_2 | 22 | PRODUCT Size | 160 bp | Primer Start | 18479 | Primer End | 18638 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGGGGTAGTGCACTCATGATA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCCATACCAACGGTACTTGTC | |||||||||||||
| Tm_F_3 | 59.013 | Size_F_3 | 21 | Tm_R_3 | 59.228 | Size_R_3 | 21 | PRODUCT Size | 142 bp | Primer Start | 18497 | Primer End | 18638 |