Primers Available
Organism:Naegleria fowleri mitochondrion
Repeat:(T)13
Start:12970 End:12982
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGGTTAATTGATTCGAGGGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGGGCAACAAGACACACAAT | |||||||||||||
| Tm_F_1 | 58.011 | Size_F_1 | 20 | Tm_R_1 | 60.823 | Size_R_1 | 20 | PRODUCT Size | 111 bp | Primer Start | 12946 | Primer End | 13056 |
| FORWARD_PRIMER_2 | |||||||||||||
| GATGGTTAATTGATTCGAGGGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGGGCAACAAGACACACAAT | |||||||||||||
| Tm_F_2 | 60.15 | Size_F_2 | 22 | Tm_R_2 | 60.823 | Size_R_2 | 20 | PRODUCT Size | 113 bp | Primer Start | 12944 | Primer End | 13056 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGGTTAATTGATTCGAGGGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGCTTCTGCTGGATTCAAAC | |||||||||||||
| Tm_F_3 | 58.011 | Size_F_3 | 20 | Tm_R_3 | 59.002 | Size_R_3 | 20 | PRODUCT Size | 135 bp | Primer Start | 12946 | Primer End | 13080 |